Composite

Part:BBa_K190036:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)

GVP with LacI regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 4347
    Illegal XhoI site found at 4035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
    Illegal NgoMIV site found at 1395
    Illegal AgeI site found at 4314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2805
    Illegal SapI.rc site found at 3961


Design Notes

Melbourne GVP cluster was used containing the 400bp repeat in gvpL.

Source

E.coli and Bacillus megaterium

References