Composite
Part:BBa_K190036:Design
Designed by: Michael Verhoeven Group: iGEM09_Groningen (2009-09-07)
GVP with LacI regulated promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 542
Illegal BamHI site found at 4347
Illegal XhoI site found at 4035 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 993
Illegal NgoMIV site found at 1395
Illegal AgeI site found at 4314 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2805
Illegal SapI.rc site found at 3961
Design Notes
Melbourne GVP cluster was used containing the 400bp repeat in gvpL.
Source
E.coli and Bacillus megaterium